Gill III Hematoxylin Solution

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Gill III Hematoxylin Solution

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SKU N/A Categories : Digital-Optimized H&E & Special Stains, IHC Compatible Histopathology Reagents (Fix to Mount)

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Gill III Hematoxylin is the most powerful and fastest stain in the Gill series. It is triple the strength of Gill I and 1.5x stronger than Gill II.
It is formulated as per Dr. G. W. Gill’s original formula, containing 6g/L Hematoxylin – the maximum concentration possible while keeping it progressive.
It is designed for High-Volume Pathology Labs, Frozen Sections, Urgent Biopsies, and Automated Tissue Stainers where staining time must be under 60 seconds.
If the lab does >500 H&E slides per day or uses an automatic slide stainer, Gill III is the appropriate variant to achieve intense nuclear staining in just 45 seconds to 1 minute.

Fastest Gill Stain – 45 Second Staining: Triple strength means fastest turnaround.
Best for Automated Stainers: Specifically designed for auto-stainers. Low viscosity, 0.2 micron filtered, high glycol content prevents drying on stainer nozzles and prevents precipitate clogging.
High-Contrast Nuclear Stain: Gives darkest blue nuclei among all progressive stains. Ideal for low-power scanning (4x). Tissue architecture, invasion, margins are visible instantly. Pathologists who like dark Harris-type H&E love Gill III.
Zero Scum, Zero Daily Filtering: Unlike Harris which forms black scum in 24 hours and blocks auto-stainer lines, Gill III has no metallic scum even after 30 days in open stainer jar. No slide artifacts.
Mercury-Free, Stable for 24 Months: Stable, safe, and consistent till last drop. No ripening needed.
Pack: 500ml, 1L, 2.5L Amber Glass/HDPE

High-Density Progressive Lake
Same proven Gill mechanism, but with triple dye load for instant saturation.

Step 1 – Maximum Lake Density: 6g/L Hematein + Aluminum Sulfate creates a very high-density Aluminum-Hematein Lake with maximum positive charge.
Step 2 – Ultra-Fast Binding: Due to high dye concentration, electrostatic binding to nuclear DNA is almost instant. Nuclear binding sites get saturated in 45 seconds.
Step 3 – Still Progressive: Despite being very strong, it is STILL progressive – it will NOT over-stain black like Harris. After 2 minutes, staining intensity plateaus. This gives a safety window.
Step 4 – Intense Blue Conversion: On washing with tap water, nuclei turn to deep intense blue-black, giving high contrast with Eosin.

Result: Very Dark Blue, High-Contrast Nucleus – Perfect for fast screening under low power (4x, 10x). Pathologist can see tissue architecture instantly.

For High-Volume Labs (>500 slides/day):

For Pathologists:

For Frozen Section Labs:

1. Rapid Intra-Operative Diagnosis: 90-second H&E protocol with Gill III is now standard in many oncology OT complexes.

Technical Specifications :

Specification	Details
Type	Triple-Strength Progressive Aluminum Hematoxylin
pH	2.0 – 2.5
Staining Time	30 sec – 1 min
Shelf Life	24 Months at RT
Grade	Histology Grade – For Automated & Manual Use

Important Note :

Gill III is a very strong stain. For delicate biopsies (endometrium, bone marrow) and for PAP cytology, reduce time to 20-30 seconds or use Gill I/II. Overstaining may mask fine chromatin if left for >2 minutes.