Alcian Blue Stain Kit

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Alcian Blue Stain Kit

pH 2.5-For Acid Mucin

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Alcian Blue Stain Kit, pH 2.5 is a histochemical staining system for the selective demonstration of acidic mucopolysaccharides and acid mucins in tissue sections. It produces a characteristic blue coloration of acidic mucin and is commonly used in histopathology for the evaluation of mucin-producing tissues and pathological changes associated with mucin accumulation.
  • Optimized for detection of acid mucins at pH 2.5
  • Demonstrates acidic mucopolysaccharides with clear blue staining
  • Suitable for routine histopathology and special-stain applications
  • Provides good contrast between mucin and surrounding tissue structures
  • Convenient kit format for standardized laboratory use
  • Suitable for paraffin-embedded tissue sections
  • Compatible with commonly used counterstaining procedures
Working Principle At pH 2.5, Alcian Blue is a cationic copper-containing phthalocyanine dye that binds electrostatically to negatively charged acidic groups present in acidic mucins and related acidic mucopolysaccharides. These components are consequently demonstrated in blue, while the selected counterstain provides contrast for the surrounding tissue structures.
  • Enables reliable histochemical demonstration of acid mucin
  • Provides distinct blue visualization for easier microscopic assessment
  • Useful for identifying and characterizing mucin distribution in tissue sections
  • Supports evaluation of mucin-producing lesions and pathological specimens
  • Helps differentiate acidic mucins from other tissue components
  • Provides a practical special-stain method for routine histopathology laboratories

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