AZF Rapid Bone Marrow Fixative
- AZF (Acetic Zinc Formalin) Rapid Bone Marrow Fixative is a next-generation, mercury-free, zinc-based fixative specially formulated for Rapid Bone Marrow Trephine Biopsy. It combines the nuclear detail of B5 fixative with the safety and IHC compatibility of 10% NBF.
- Designed for same-day diagnosis, it fixes, partially decalcifies, and preserves marrow in short TAT.
- It is 100% Mercury-Free, No Picric Acid.
- It is the ideal fixative for labs wanting B5-quality morphology without hazardous mercury waste and without losing IHC / Molecular.
- Rapid Fixation Technology: Acetic acid + Zinc synergy accelerates penetration to 3mm/hour – 3X faster than NBF.
- Zinc-Based Nuclear Fixation: Zinc Chloride preserves chromatin like mercuric chloride in B5, but non-toxic.
- Mild Decal Action: Built-in weak acid gently softens trabecular bone during fixation – reduces decalcification time by 50%.
- pH Optimized 5.0-5.5: Acetic buffered pH enhances nuclear membrane sharpness for dysplasia assessment.
- Ready-to-Use 60mL Vial ( On demand ): Pre-filtered, pre-mixed – just drop trephine core. 18 months shelf life.
- Universal Compatibility: One fixative for H&E, Reticulin, Iron, IHC, and FISH – No need for separate Zenker’s.
(Dual Action Mechanism)
- AZF works by Coagulative + Cross-Linking Dual Fixation :
- Step-by-Step :
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Acetic Acid (Coagulative): Rapid penetration -> Swells nucleus -> Opens chromatin
Zinc Chloride (Ionic Bonding): Zn++ binds nucleoproteins -> Locks chromatin pattern
Formaldehyde (Cross-linking): Stabilizes cytoplasm and marrow architecture
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0-15 min – Rapid Penetration: Acetic acid penetrates dense bone core instantly, prevents central autolysis.
15-60 min – Zinc Chromatin Lock: Zinc ions bind DNA-histone complexes, preserving fine chromatin, nucleoli, and Auer rods exactly like B5.
60-120 min – Architecture Fixation: Formaldehyde cross-links stroma and fat – intact marrow cellularity and fibrosis pattern.
- For Pathologist – B5 Quality Without Mercury :
- For Histotechnologist :
- For Lab / Hospital :
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Superior Nuclear Detail: Crisp chromatin for MDS, Leukemia blasts, megaloblasts. Easily identifies dysplasia, Auer rods, ring sideroblasts.
Excellent Morphology Score: MF grading, cellularity, and M:E ratio 20% clearer than NBF alone.
100% IHC Retention: Unlike B5 which destroys CD34, CD117, CD138 – AZF preserves all Lymphoid/Myeloid markers. Validated for CD34, CD117, MPO, CD138, CD20, CD3, CD61.
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Faster TAT
Easy Sectioning: Bone is pre-softened, cuts smooth 2µm ribbons – No shattering, no chattering.
No Formalin Pigment: Acetic acid prevents acid hematin – No confusion with malaria / hemosiderin.
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Mercury-Free & Safe: No B5 mercuric chloride disposal cost. No EPA hazardous waste manifest needed.
CAP & WHO Compliant: Meets WHO recommendation for mercury-free marrow fixation.
Cost & Time Saving: 60% reduction in Turn Around Time (TAT) – Improves biopsy reporting revenue.
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Bone Marrow Fixatives for IHC
Standard Bone Marrow Fixative
Price range: ₹1,000.00 through ₹2,000.00 Select options This product has multiple variants. The options may be chosen on the product page
