Fite’s Stain Kit
Fite’s Stain is a modified acid-fast staining method designed for the histological demonstration of Mycobacterium leprae and Nocardia in paraffin-embedded tissue sections. The method uses an oil-containing deparaffinization step to help preserve the relatively delicate acid-fastness of M. leprae.
- Special stain for Mycobacterium leprae and Nocardia
- Suitable for formalin-fixed, paraffin-embedded tissue sections
- Modified acid-fast technique using xylene–peanut oil deparaffinization
- Helps preserve the acid-fast characteristics of delicate organisms
- Carbol fuchsin provides strong red/magenta staining of acid-fast organisms
- Methylene blue provides a contrasting blue background
- Suitable for routine histopathology laboratories
- Can be used for both Leprosy and Nocardia staining protocols, with appropriate differentiation conditions.
- The waxy, lipid-rich cell wall of acid-fast organisms retains carbol fuchsin despite controlled decolorization. In Fite staining, xylene–peanut oil is used for deparaffinization to reduce loss of acid-fastness of M. leprae. After staining with carbol fuchsin, controlled differentiation removes stain from the surrounding tissue while acid-fast organisms remain red. Methylene blue provides the contrasting background.
- Typical result:
-
1. Leprosy bacilli / acid-fast organisms: Bright red to magenta
2. Nuclei/background: Blue
- Optimized for delicate acid-fast organisms: Particularly useful for demonstrating M. leprae, whose acid-fastness can be compromised by conventional deparaffinization.
- Clear organism-to-background contrast: Red/magenta bacilli against a blue background facilitates microscopic recognition.
- Useful in tissue diagnosis: Supports histopathological evaluation of suspected leprosy and Nocardia infection.
- Paraffin-section compatible: Designed for routine FFPE histology specimens.
- Convenient laboratory workflow: Ready-to-use kit formats can provide standardized staining reagents and reduce individual reagent preparation.
- Suitable for microscopic confirmation: Organisms can be examined at high magnification, including oil-immersion microscopy when required.
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