Eosin Y Stain Solution 0.5%(w/v) Alcoholic

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Eosin Y Stain Solution 0.5%(w/v) Alcoholic

Light Eosin for delicate Biopsies & Cytosmears

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Standard 1% Eosin is too strong for delicate tissues. It over-stains and hides nuclear details. Light Eosin 0.5% is a specially diluted, gentle formulation designed for thin, hypocellular, and delicate samples. It gives a soft, transparent, baby-pink that never masks the nucleus. This is why cytologists, renal biopsy, and transplant biopsy experts love it. If standard eosin is a thick marker, Light Eosin is a fine-liner pen.
  • True 0.5% w/v Low Concentration: 50% lighter than routine eosin -Prevents overstaining by design
  • Soft Baby Pink Shade: Light, transparent, pastel pink – Never dark or red
  • Alcoholic & Gentle: Optimized 70% ethanol base – No tissue detachment for small biopsies
  • Nuclear Friendly: Very low cytoplasmic density – Nuclei remain dominant and crisp blue
  • Prevents Chromatin Masking: Critical for cytosmears where nuclear details = diagnosis
  • Ready-to-Use 0.22 Micron Filtered: No filtering, no dilution, no precipitate on delicate cells
  • pH Balanced at 5.0-5.2: Slightly higher pH than 1% eosin for lighter, softer binding
  • Economical for Cyto Use: Low consumption – 500 ml does >2000 cytosmears
  • Pack Sizes: 500 ml, 1 Liter
In delicate biopsies & cytosmears, the challenge is opposite – that it requires LESS eosin, not more.

  • Problem with 1% Eosin: Heavy eosin saturates thin cytoplasm, creates dark pink veil over nucleus. Pathologist struggles to see chromatin.
  • 0.5% Light Eosin: Contains half the dye molecules. It stains cytoplasm just enough to give orientation, but stays transparent.
  • Result – Nucleus Dominant H&E:Cytoplasm: Very Light, Transparenn Baby Pink Nuclei:vSuper-Crisp, Dark Blue 100% Visible RBCs: Light Orange – Not distracting
  • Principle: Less is More. Light cytoplasmic counterstain = Maximum nuclear details.
  • 0.5% Light Concentration , No Overstaining Ever. Even if technician forgets slide for 3-4 min, it stays light. Foolproof for busy cyto labs.
  • Transparent Baby Pink, Nuclear Details Pop Out. Chromatin, nucleoli, inclusions – crystal clear. Critical for Pap, FNAC, Fluid Cytology.
  • Ideal for Tiny Biopsies, No Loss of Small Fragments. Renal biopsy, gastric biopsy, endoscopic biopsy – 2mm tissue needs light pink only.
  • Best for Cytosmears, Better Than 1% for Cyto. Pap smear, FNAC, Bronchial wash, Urine, Body fluids – Light pink is textbook standard.
  • Alcoholic Base, No Cell Loss. Aqueous eosin detaches cells from slide. Alcoholic version protects cellularity.
  • Keeps Background Clean, White Background Stays White. Mucin, colloid, fluid background does not take pink – Clean reporting.
  • Technical Specifications :
  • Specification Details
    CAS No. 17372-87-1
    pH 5.0 – 5.2 ± 0.2
    Appearance Clear, Light Orangish-Pink Solution
    Staining Time 1.5 to 3 minutes (Longer than 1% because it is lighter)
    Storage 15-30°C, away from direct sunlight
    Stability 24 Months

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