Gill II Hematoxylin Solution

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SKU N/A Categories : Digital-Optimized H&E & Special Stains, IHC Compatible Histopathology Reagents (Fix to Mount)

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Gill II Hematoxylin is the most widely used H&E Hematoxylin in modern histology labs worldwide. It is double the strength of Gill I and formulated as per Dr. G. W. Gill’s formula.
It is a progressive, aluminum-based, mercuric oxide-free nuclear stain. It is the perfect balance – Strong enough to stain all tissues (Liver, Colon, Breast, Prostate) in just 2 minutes, but light enough to still be progressive (No differentiation needed).
If Gill I is for Cytology, Gill II is for Histology.
99% of NABL accredited labs and large corporate pathology chains in India have now shifted from Harris to Gill II because Harris causes over-staining, metallic scum, and batch-to-batch variation.

Perfect Strength for H&E: Contains 4.0g/L Hematoxylin – the optimal concentration. Gill I is too light for histology (needs 4 min), Gill III is too dark (risk of opaque nuclei). Gill II is the sweet spot.
True Progressive – No Acid Alcohol Needed: Eliminates differentiation step completely. Saves cost of Acid-Alcohol, saves technician time, and eliminates 90% of H&E errors (faded nuclei due to over-differentiation).
Zero Metallic Scum & Precipitate: Formulated with high Ethylene Glycol (250ml/L) and stable oxidizer. Unlike Harris which forms black metallic film on stain jar and black dots on slides, Gill II is clean till last drop. No daily filtering.
Crystal Clear Nuclear Chromatin: Gives transparent staining – pathologists can see chromatin pattern, nucleoli, mitosis, nuclear grooves very clearly. Critical for cancer grading (Breast, Prostate). Harris gives opaque black nuclei.
Mercury-Free & Eco-Friendly: Contains Sodium Iodate as oxidizer, not Mercuric Oxide. Safe for technicians and for disposal. Complies with NABL & Pollution Control norms.
2-Year Shelf Life: Chemically ripened formula is ultra-stable. Harris expires in 6-8 months. Gill II is stable for 24 months at Room Temperature. No loss of staining intensity.

Double-Strength Progressive Lake
Same mechanism as Gill I, but with double dye concentration for faster histology workflow.

Step 1 – Lake Formation: 4g/L Hematein (oxidized Hematoxylin) + Aluminum Sulfate forms intense Aluminum-Hematein Lake.
Step 2 – Progressive Saturation:This cationic lake binds to anionic DNA phosphate groups in nucleus. Because concentration is double of Gill I, saturation is achieved in 90 seconds to 2 minutes for 4-micron sections.
Step 3 – Self-Limiting: Once all DNA binding sites are occupied, no further dye uptake occurs. So even if technician leaves slide for 4 minutes, it will NOT over-stain. This is the biggest advantage over Harris.
Step 4 – Blueing: Simple tap water wash at pH >7 converts red-purple lake to deep blue-purple in nucleus.

Result: Sharp Dark Blue Nucleus, clear chromatin, no cytoplasmic staining.

Specification	Details
Type	Progressive Aluminum Hematoxylin
Concentration	Double strength of Gill I
pH	2.0 – 2.5
Color	Deep Purple
Staining Time	1-2 min.
Shelf Life	24 Months at RT
Pack	500ml, 1L, 2.5L Amber HDPE/Glass Bottle
Grade	Histology Grade
Pair With	Perfect results with 1% Eosin Y Alcoholic, 1% Eosin Y Aqueous, Scott’s Bluing Reagent, and Xylene-Free Clearing Agent.