May-Grunwald-Giemsa(MGG) Stain Kit

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May-Grunwald-Giemsa(MGG) Stain Kit

For Hematology & Cytology

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SKU N/A Category : Routine & Special Hematology Stains

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May-Grünwald-Giemsa (MGG) Stain Kit is a polychromatic Romanowsky-type staining system designed for differential staining of peripheral blood films, bone marrow smears and suitable air-dried cytological preparations. It provides clear differentiation of nuclei, cytoplasm, erythrocytes, leukocyte granules and other cellular components, supporting detailed morphological examination under light microscopy.

Classic May-Grünwald-Giemsa Romanowsky staining system
Suitable for routine hematology and cytology
Designed for blood and bone marrow smears
Suitable for appropriate air-dried cytological preparations, including FNAC and body-fluid preparations
Provides distinctive nuclear and cytoplasmic colour differentiation
Enhances visualization of leukocyte granules and cellular inclusions
Produces characteristic blue, purple, pink and orange-red Romanowsky colour reactions
Methanol-containing May-Grünwald reagent provides fixation of suitable air-dried smears
Compatible with conventional manual staining procedures
Suitable for differential leukocyte and cellular morphology assessment

MGG staining combines May-Grünwald and Giemsa staining systems. May-Grünwald reagent contains eosin and methylene blue in an alcoholic medium, while Giemsa contributes methylene-blue derivatives including azure dyes together with eosin.
The basic thiazine dyes, including methylene blue and azure B, bind preferentially to acidic cellular components such as DNA and RNA, producing blue to violet staining. Eosin Y, an acidic dye, interacts with basic protein-rich structures and produces pink to orange-red staining. The interaction of azure B and eosin produces the characteristic Romanowsky effect, providing enhanced differentiation of nuclear chromatin, cytoplasm and leukocyte granules.
The final staining quality is strongly influenced by buffer pH, dye composition, fixation, staining time and washing conditions. A working pH around 6.5–6.8 is commonly used for MGG staining.

Excellent Cellular Differentiation

Provides contrasting staining of nuclei, cytoplasm, granules and other cellular structures for detailed morphological assessment.

Enhanced Nuclear Detail

Produces blue-violet to purple staining of nuclear chromatin, helping reveal nuclear morphology and chromatin patterns.

Clear Cytoplasmic Contrast

Provides distinct cytoplasmic colour differentiation, supporting assessment of cellular maturation and morphology.

Effective Leukocyte Differentiation

Facilitates morphological differentiation of lymphocytes, monocytes, neutrophils, eosinophils and basophils through characteristic cytoplasmic and granule staining.

Detailed Granule Visualization

Enhances the distinction of leukocyte granules, contributing to differential cellular identification.

Suitable for Bone Marrow Examination

Provides the cellular and granule detail required for morphological assessment of bone marrow smears.

Cytology Applications :

Useful for appropriate air-dried cytological preparations, including fine-needle aspiration and selected body-fluid specimens.

Characteristic Romanowsky Colouration

Produces the characteristic blue, violet, pink and orange-red colour spectrum associated with MGG staining.

Reproducible Routine Staining

When used with controlled fixation, buffer pH, staining time and washing conditions, the system supports consistent staining quality.

Typical Applications :