New Methylene Blue(NMB) Stain Kit
New Methylene Blue (NMB) Stain Kit is a supravital staining system designed for the microscopic demonstration and enumeration of reticulocytes and for the detection of Heinz bodies and selected erythrocyte inclusions. It highlights residual ribosomal RNA in immature red blood cells as blue reticulofilamentous material, allowing reticulocytes to be differentiated from mature erythrocytes.
- Supravital stain for reticulocyte examination
- Designed for reticulocyte counting
- Suitable for demonstration of Heinz bodies
- Highlights residual RNA in immature erythrocytes
- Produces distinct blue reticulofilamentous material in reticulocytes
- Helps differentiate reticulocytes from mature erythrocytes
- Suitable for fresh or appropriately anticoagulated blood specimens
- Supports manual microscopic reticulocyte estimation
- Useful for assessment of erythrocyte inclusions
- Suitable for routine hematology laboratory applications
- New Methylene Blue is a supravital dye that is applied to unfixed blood cells. It interacts with residual ribosomal RNA and other reticulofilamentous material present in immature red blood cells. Following incubation, the residual RNA becomes visible as blue granular or filamentous material, while mature erythrocytes, which have lost their residual RNA, show little or no reticulofilamentous staining.
- The stained preparation can be examined microscopically and reticulocytes can be expressed as a percentage of the red-cell population. NMB may also demonstrate Heinz bodies, which appear as pale blue intracellular inclusions, although dedicated Heinz-body stains such as methyl violet may provide stronger visualization when specifically required.
- Clear Reticulocyte Visualization
- Supports Reticulocyte Counting
- Demonstration of Heinz Bodies:
- Distinction from Mature RBCs
- Assessment of Erythropoietic Activity
- Useful for Erythrocyte Inclusion Studies
- Routine Hematology Application:
- Typical Applications :
- Highlights residual RNA as blue reticulofilamentous material, facilitating recognition of immature red blood cells.
- Provides a practical microscopic method for estimating the percentage of reticulocytes in a blood specimen.
- Can demonstrate Heinz bodies as pale blue intracellular inclusions in supravitally stained erythrocytes.
- Mature erythrocytes lack significant residual RNA and therefore show substantially less reticulofilamentous staining.
- Reticulocyte enumeration provides information about the bone marrow’s production of new erythrocytes and the response to increased red-cell demand.
- Supports microscopic examination of selected red-cell inclusions, including Heinz bodies and HbH-related inclusions under appropriate staining conditions.
- Suitable for laboratories performing manual supravital reticulocyte examinations and erythrocyte morphology studies.
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1. Manual reticulocyte counting
2. Reticulocyte morphology
3. Demonstration of Heinz bodies
4. Investigation of erythrocyte inclusions
5. Assessment of erythropoietic activity
6. Selected investigations of HbH inclusions
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