Congo Red Stain Kit
Amyloid Stain kit
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Categories : Digital-Optimized H&E & Special Stains, IHC Compatible Histopathology Reagents (Fix to Mount)
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Congo Red Stain Kit is a histopathology staining system designed for the demonstration and identification of amyloid deposits in tissue sections. Congo Red binds selectively to amyloid and produces a characteristic red to deep pink staining under bright-field microscopy. When examined using polarized light, Congo Red–stained amyloid demonstrates characteristic birefringent/anomalous optical colours, classically described as apple-green birefringence.
- Designed for reliable demonstration of amyloid deposits in tissue sections
- Produces distinct red to deep pink staining of amyloid under bright-field microscopy
- Enables evaluation of Congo Red–positive deposits under polarized light
- Suitable for routine histopathology and biopsy examination
- Clear contrast between amyloid deposits and surrounding tissue structures
- Convenient kit format for standardized laboratory staining
- Suitable for use on formalin-fixed, paraffin-embedded tissue sections
Congo Red dye binds to the organized fibrillar structure of amyloid deposits. The bound dye molecules become aligned along the amyloid fibrils, producing characteristic optical properties when viewed between crossed polarizers. Amyloid deposits can therefore be assessed by combining their Congo Red staining characteristics with polarized-light examination.
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1. Applications:
- Detection of amyloid deposits in tissue biopsies
- Evaluation of suspected systemic and localized amyloidosis
- Examination of kidney, liver, heart, spleen and other tissue specimens
- Demonstration of amyloid in diagnostic histopathology
- Research and teaching applications involving amyloid deposition
- Facilitates histochemical demonstration of amyloid
- Provides strong visual contrast for easier microscopic assessment
- Polarized-light examination provides an important confirmatory feature for Congo Red–positive amyloid
- Supports routine diagnostic evaluation of amyloid deposition
- Convenient and standardized staining format for pathology laboratories
Microscopy Under ordinary light microscopy, amyloid typically appears red to deep pink following Congo Red staining. Under appropriately configured polarized-light microscopy, Congo Red–stained amyloid may show characteristic green and other anomalous birefringent colours.
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