SlideBond TM Adhesive
- SlideBond™ Adhesive is the gold-standard, covalent-bonding tissue adhesive for labs doing Immunohistochemistry (IHC), In-Situ Hybridization (ISH), and Fluorescence In-Situ Hybridization (FISH).
- Normal slides and even “charged” slides fail in harsh IHC / FISH protocols because they use weak electrostatic attraction. Expensive biopsy tissue washes off during HIER (pressure cooker boiling), enzyme digestion (Proteinase K), and high-temperature hybridization (90°C).
- SlideBond™ creates a permanent, chemical covalent bond between the glass slide and the tissue section. Once bonded, the section will NEVER detach, even after 4 hours of boiling and 24 hours of hybridization.
Why Labs Prefer SlideBond™ Over Poly-L-Lysine and Charged Slides?
- Poly-L-Lysine: Only ionic bond, washes off in alkaline retrieval (pH 9.0) and during FISH. Also causes high non-specific background.
- Commercial Charged Slides: Expensive, coating thickness varies batch-to-batch, and lose charge after 6 months.
- SlideBond™ Adhesive: Covalent Si-O-Si bonding. Withstands pH 1 to pH 11, temperature up to 120°C, Proteinase K, Pepsin, and Formamide. IHC-compatible quality with a more competitive cost per slide, and coating lasts 2 years.
- FISH / ISH / IHC Validated: Specifically optimized to withstand Pressure Cooker HIER (pH 6.0 & pH 9.0), Microwave retrieval, PT-Link, Proteinase K (15 min), Pepsin (30 min), and Formamide hybridization at 80-90°C.
- Zero Background: Unlike Poly-L-Lysine, SlideBond is molecularly thin (monolayer) and does NOT pick up Hematoxylin or DAB, so background is crystal clear.
- Ready-to-Coat Concentrate: 99% Pure SlideBond liquid – 50ml bottle makes 2.5 Liters of working solution = Coats 2500 slides. Ultra-economical.
- Universal Compatibility: Works with ALL tissues – Bone decalcified with acid, brain, fatty breast, necrotic tumor, tiny endoscopic biopsies that detach easily.
- Superior to Pre-Coated Slides: Freshly coated slides give 10x more adhesion than 1-year-old company-coated charged slides.
Covalent Bonding Chemistry
1. This is a 3-step chemical reaction, not just stickiness :
1. This is a 3-step chemical reaction, not just stickiness :
- Step 1 – Activation: Glass slide surface has Silanol groups (Si-OH). When dipped in SlideBond, its Ethoxy groups (-OCH2CH3) react with Si-OH.
- Step 2 – Silanization: It forms a strong, irreversible Siloxane Bond (Si-O-Si) with the glass. One end is now permanently anchored to the slide.
- Step 3 – Amine Exposure: Amine group forms strong electrostatic and covalent cross-links with negatively charged tissue proteins (Carboxyl groups of tissue sections).
- For IHC Lab :
- For FISH / ISH / CISH Lab :
- For Histology Lab :
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Never lose small biopsies again. Solves the #1 complaint: “Breast core / Prostate chip / GI biopsy washed off during retrieval.” Saves repeat biopsy requests and patient recall. Ideal for ER, PR, HER2 where re-cutting is mandatory.
- This is the ONLY recommended adhesive for HER2 FISH, EBV ISH, ALK FISH. Sections stay intact after overnight hybridization at 37°C and harsh post-wash buffers. No more floating tissue during DAPI counterstain.
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1. Cost Saving: Significantly lower cost than commercial charged slides.
2. Long Shelf Life: SlideBond Solution is stable for 3 years at 4-25°C. Coated slides stable for 2 years in dry box.
3. Better Microtomy: SlideBond treated slides are slightly less hydrophilic, so sections flatten better and don’t wrinkle during pickup from water bath.
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