Sudan Black B (SBB) Stain Kit

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For Diagnosis of Acute Leukaemia (AML vs ALL)

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SKU N/A Category : Routine & Special Hematology Stains

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Sudan Black B (SBB) Stain Kit is a cytochemical staining system designed for the demonstration of lipid-rich cytoplasmic granules in hematopoietic cells. It is particularly useful as an adjunctive stain in the cytochemical evaluation of acute leukemias, where positive staining supports myeloid differentiation and can assist in distinguishing Acute Myeloid Leukemia (AML) from Acute Lymphoblastic Leukemia (ALL).
SBB staining generally parallels the staining pattern of myeloperoxidase (MPO), with myeloid blasts typically showing dark granular positivity, whereas lymphoblasts are generally negative. However, SBB is less specific than MPO and rare SBB-positive ALL cases have been reported; therefore, results should be interpreted together with morphology, immunophenotyping and other laboratory findings.

Sudan Black B is a lipid-soluble dye that has an affinity for lipid-containing cellular structures. In hematopoietic cells, it stains lipid-rich components associated with primary and secondary granules of granulocytic cells and lysosomal granules of monocytic cells.
Following staining, myeloid cells and myeloid blasts containing suitable cytoplasmic granules develop dark brown to black granular deposits. Lymphoid cells are generally negative. This staining pattern can therefore provide evidence of myeloid differentiation and support the cytochemical distinction between AML and ALL.

Provides useful cytochemical evidence of myeloid differentiation when evaluating acute leukemia.

Highlights lipid-containing cytoplasmic granules with a characteristic dark granular reaction.

Can demonstrate myeloid differentiation in certain immature or poorly differentiated leukemic blasts.

Produces a readily recognizable brown-black granular staining pattern for microscopic assessment.

SBB provides a staining pattern broadly similar to MPO and can be used as a complementary cytochemical investigation.

Designed for cytochemical evaluation of appropriately prepared peripheral blood and bone marrow smears.

Adds cytochemical information to routine Romanowsky-stained morphology during acute leukemia evaluation.

Provides a conventional cytochemical approach for laboratories performing hematological lineage investigations.