Modified Gram Stain Kit
- Modified Gram Stain Kit is a differential staining system designed for the demonstration and differentiation of Gram-positive and Gram-negative bacteria in direct smears and body-fluid specimens. The modified staining procedure provides good bacterial contrast against the cellular and specimen background, supporting microscopic evaluation of bacterial morphology, distribution and Gram reaction.
- It is suitable for routine microbiological and cytological examination of appropriately prepared specimens where bacterial infection is suspected.
- Modified Gram staining system for direct smears and body fluids
- Demonstrates Gram-positive and Gram-negative bacteria
- Produces characteristic differential colour reactions
- Provides good bacterial-to-background contrast
- Suitable for appropriately prepared body-fluid smears
- Useful for direct clinical specimen examination
- Helps demonstrate bacterial morphology and distribution
- Suitable for routine laboratory microscopy
- Compatible with conventional manual staining procedures
- Designed for rapid microscopic assessment of stained smears
- Gram staining is based on the differential retention of a crystal violet–iodine complex by bacterial cell walls. Following primary staining and iodine treatment, the decolorization step differentiates bacteria according to their cell-wall characteristics.
- Gram-positive bacteria generally retain the primary dye complex and appear blue to violet/purple, while Gram-negative bacteria are decolorized and subsequently take up the counterstain, appearing pink to red.
- The modified staining sequence is optimized for smear and body-fluid preparations to provide clear bacterial visualization while maintaining sufficient contrast with the surrounding cellular material.
- Clear Gram Differentiation
- Good Bacterial Visibility
- Useful for Body-Fluid Examination
- Supports Bacterial Morphology Assessment
- Rapid Microscopic Assessment
- Suitable for Direct Smears
- Enhanced Cellular Contrast
- Routine Laboratory Application
- Typical Applications :
- Typical Staining Appearance :
- Provides characteristic colour differentiation between Gram-positive and Gram-negative organisms.
- Enhances visualization of bacteria against the cellular and specimen background.
- Suitable for direct smears prepared from appropriately collected body-fluid specimens.
- Helps demonstrate bacterial size, shape, arrangement and Gram reaction.
- Provides a practical staining approach for preliminary microscopic evaluation of suspected bacterial infection.
- Designed for appropriately prepared clinical smears without requiring tissue processing.
- Counterstaining provides useful contrast between microorganisms and surrounding cells and background material.
- Suitable for microbiology, cytology and clinical laboratory workflows involving direct smear examination.
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1. Body-fluid smears
2. Direct clinical specimen smears
3. Pus and exudate smears
4. Respiratory specimen smears
5. Wound and discharge specimens
6. Cytological preparations requiring bacterial demonstration
7. Demonstration of Gram-positive bacteria
8. Demonstration of Gram-negative bacteria
9. Preliminary microscopic assessment of bacterial morphology
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Gram-positive bacteria: Blue to violet/purple
Gram-negative bacteria: Pink to red
Background/cellular components: Contrasting counterstain, depending on the specimen and staining protocol
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