PAS-D Stain Kit
PAS-D Stain Kit is a specialized histopathology staining system combining Periodic Acid–Schiff (PAS) staining with diastase digestion. It is used to demonstrate PAS-positive, diastase-resistant substances in tissue sections and is particularly useful for differentiating glycogen from other PAS-positive materials.
- Complete kit for PAS-D histochemical staining
- Combines diastase digestion with PAS staining
- Demonstrates diastase-resistant PAS-positive material
- Useful for routine histopathology and special-stain applications
- Produces distinct magenta to red-purple PAS-positive staining
- Suitable for formalin-fixed, paraffin-embedded (FFPE) tissue sections
- Designed for consistent and reproducible staining
- Convenient workflow with ready-to- use or appropriately prepared reagents
The PAS-D technique consists of two main stages:
- Diastase digestion: Tissue sections are treated with diastase to enzymatically remove glycogen.
- PAS staining: Periodic acid oxidizes suitable tissue carbohydrates, producing aldehyde groups that react with Schiff reagent to generate a characteristic magenta coloration.
- Comparison of the PAS and PAS-D reactions: helps determine whether a PAS-positive substance is diastase-sensitive (such as glycogen) or diastase-resistant.
- Helps differentiate glycogen from diastase-resistant PAS-positive substances.
- Enhances the diagnostic utility of conventional PAS staining.
- Provides clear visualization of carbohydrate-rich tissue components
- Useful in the evaluation of selected fungal organisms, basement membranes, mucosubstances and other PAS-positive structures
- Supports comparative interpretation between PAS and PAS-D sections
- Convenient for routine histopathology laboratories
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