Eosin Y-Phloxine B Stain Solution
Bright Red Variant for High Contrast H&E
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Categories : Digital-Optimized H&E & Special Stains, IHC Compatible Histopathology Reagents (Fix to Mount)
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Eosin Y- Phloxine B is a premium dual-dye formulation where Phloxine B (a brilliant red xanthene dye) is added to Eosin Y. While standard eosin gives a standard pink, Phloxine B imparts an intense, fiery red-orange contrast that makes cytoplasm, RBCs, and collagen pop under the microscope. Preferred for high-cellular biopsies, oncology specimens, and digital slide scanning.
- Dual-Dye Technology: Combination of Eosin Y (Yellowish) + Phloxine B (Bluish-Red) for extended color spectrum
- Brilliant Red Shade: Gives 3-Tone Eosinophilia – Pink, Orange-Red & Fiery Red vs Single Pink in routine eosin
- Phloxine B Enhanced: 5-10% Phloxine B w.r.t Eosin – Scientifically optimized ratio
- Alcoholic Base: 70-90% Ethanol/Methanol formulation – Rapid penetration, no overstaining
- High-Contrast Formulation: Specifically for WSI / Digital Pathology scanners
- Ready-to-Use 1% w/v: No dilution, no filtering required
- ISO 13485 Manufactured: Batch to batch consistency for NABL labs
Bright Red Variant works on differential eosinophilia principle:
- Eosin Y – Stains general cytoplasm and muscle in light pink-orange.
- Phloxine B – Being more acidic and larger molecule, it selectively binds to highly eosinophilic structures with high affinity.
- Result – 3 Color Contrast:
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General Cytoplasm: Light Pink
Collagen / Muscle / Keratin: Deep Red-Orange
RBCs / Eosinophils / Paneth Cell Granules: Fiery Brilliant Red
- For Pathologist:
- For Lab Owner:
- For Teaching Labs:
- Technical Specifications :
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Better Diagnosis in Cancer: Tumor keratin, necrosis, and cell borders are much clearer in SCC, Breast, Cervix.
Easy Hemorrhage Detection: RBCs glow, you won’t miss vascular invasion.
No Confusion: Muscle vs Collagen vs Keratin – all different reds.
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Wins Digital Pathology: Scanned slides look stunning on monitor. Perfect for Morphle, Leica, Philips scanners and AI.
Saves Special Stain Cost: In many liver / skin biopsies, you can avoid Trichrome just to see collagen.
30% Faster: Stains in 30 sec. Increases daily throughput.
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Students Learn Faster: 3 colors are easier to teach than 1 pink.
Projector Ready: Even from last bench, slide looks contrasty.
| Specification | Details |
|---|---|
| Composition | Eosin Y + Phloxine B in Alcoholic Solution |
| pH | 4.8 ± 0.2 (With Glacial Acetic Acid) |
| Color | Clear Dark Reddish-Orange Solution |
| Staining Time | 30 sec – 90 sec |
| Compatibility | All Hematoxylins (Harris, Mayer, Gill) |
| Shelf Life | 24 Months |
| Storage | 15-30°C |
| Pack Size | 500ml, 1L, 5L |
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