Modified Mayer’s Hematoxylin Solution
- Modified Mayer’s Hematoxylin is an alcohol-free, mercury-free, progressive nuclear counterstain specially modified for Immunohistochemistry (IHC), Special Stains, and Routine H&E.
- Original Mayer’s formula (1903) was too weak and unstable. We have modified it by increasing Hematoxylin concentration and adding stabilizers to give a stronger, stable stain that gives light blue transparent nuclei – without masking the brown DAB signal of IHC.
- This is the #1 Counterstain used worldwide for IHC – Recommended in all DAKO, Ventana, Leica, Biocare IHC kits.
- Why Every IHC Lab Needs Modified Mayer’s ?
- It is also used for:
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1. If you use Harris or Gill II for IHC counterstain, DAB (brown) + Dark Blue Nucleus = Blackish nucleus. Pathologist cannot see DAB properly.
2. Modified Mayer’s gives VERY LIGHT blue nucleus, so Brown DAB stands out clearly.This is why it is called IHC Counterstain.
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1. IHC Counterstain for ER, PR, HER2, Ki67, p63, CK
2. Routine H&E for delicate biopsies
3. Special Stains Counterstain – PAS, Alcian Blue, Southgate Mucin
- #1 IHC Counterstain Worldwide: Specifically designed for IHC. Light blue nuclear stain never masks brown DAB. Makes ER, PR, HER2 scoring easy and NABL compliant.
- 100% Alcohol-Free & Aqueous: Modified Mayer’s is fully aqueous. So it NEVER dissolves DAB precipitate or causes DAB fading. DAB remains dark brown.
- Progressive – No Differentiation: No acid-alcohol needed. This is critical for IHC – acid alcohol detaches IHC sections easily and damages tissue. Saves time and saves slides.
- No Background Staining: Gives absolutely zero cytoplasmic staining. Only nucleus stains light blue. Cytoplasm remains white/clear, so brown DAB signal is very prominent.
- Mercury-Free & Stable: Chemically ripened with Sodium Iodate. No Mercuric Oxide. 18 months shelf life at RT. No metallic scum, no daily filtering.
- Dual Use – IHC + H&E: Can be used as gentle H&E hematoxylin for delicate biopsies where Gill II/Harris is too dark. One bottle, two uses.
- Pack: 100ml, 500ml, 1L HDPE Amber plastic/Glass Bottle
Gentle Progressive Counterstaining
- Step 1 – Pure Aqueous Lake: Hematein + Aluminum Ammonium Sulfate forms a light, aqueous Aluminum-Hematein lake. No alcohol or ethylene glycol, so it never interferes with IHC antigen-antibody-DAB complex.
- Step 2 – Light Progressive Binding: Low concentration (1g/L Hematoxylin) binds slowly and lightly to nuclear DNA. It stains only chromatin, not over-stains.
- Step 3 – Perfect Contrast with DAB: In IHC, DAB gives brown cytoplasmic / nuclear signal. If counterstain is dark blue, brown + dark blue = black, difficult to read. Modified Mayer’s light blue + brown = excellent contrast. Pathologist can easily score ER/PR intensity and %.
- Step 4 – No Differentiation Needed: Progressive stain – washes out cleanly in tap water, no acid-alcohol needed, so delicate IHC tissue never detaches. Result: Light Transparent Blue Nucleus + Crisp Brown DAB – Perfect for IHC Scoring.
- For IHC Lab & Pathologists:
- For Lab Owners:
- Technical Specifications:
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1. Perfect ER/PR/HER2 Scoring: Light blue nuclei allows accurate Allred scoring for ER/PR. Dark hematoxylin (Harris) makes scoring difficult and leads to wrong % reporting. CAP/ASCO guidelines recommend Mayer’s type light counterstain for breast IHC.
2. NABL & CAP Compliant IHC: All NABL auditors ask for light hematoxylin counterstain for IHC. Using Harris for IHC is marked as non-compliance. Modified Mayer’s makes you compliant.
3. Zero DAB Masking: Brown DAB signal remains 100% visible, even weak 1+ positivity is not hidden.
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1. Low Cost IHC Counterstain: Ready-to-use, no dilution.
2. Reduces IHC Repeat Rate: No acid differentiation = No section loss. IHC slides are costly.
3. 18 Months Stability: Long shelf life, no wastage. Harris expires in 6 months, forms scum. Mayer’s lasts long.
| Specification | Details |
|---|---|
| Type | Progressive Aqueous Aluminum Hematoxylin |
| pH | 2.8 – 3.2 |
| Staining Intensity | Light / Delicate |
| Staining Time | 45 sec for IHC, 4-5 min for H&E |
| Shelf Life | 18 Months at RT |
| Pack | 100ml, 500ml, 1L HDPE Amber Plastic/ Glass Bottle |
| Grade | IHC Counterstain & Histology Grade |
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