Isopropyl Alcohol

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Isopropyl Alcohol

IHC Compatible

In Stock

SKU N/A Categories : IHC Compatible Histopathology Reagents (Fix to Mount), IHC Compatible Laboratory Solvents

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This is a specially formulated, high-purity dehydrating agent designed for modern histopathology where morphology, IHC antigenicity, and molecular integrity must be preserved. Unlike standard industrial alcohol, it is purified, methanol-free, low-aldehyde, and validated for immunohistochemistry.
It is the most popular and widely used dehydrating agent in histology – proven to replace water gradually without distortion of delicate cells.
It uses approved IHC-safe denaturants that do not interfere with epitope binding. It provides the exact dehydrating properties of absolute ethanol while being cost-effective and regulatory compliant.
It is completely miscible with water and xylene substitutes, allowing seamless transition from fixation to paraffin infiltration.

IHC & Molecular Validated: Low aldehyde <5 ppm, no methanol, preserves antigenicity for LH, GM130, TGN38, and routine markers, plus nucleic acid stability
Optimal Dehydration: Dehydrates rapidly without considerable shrinkage, no excessive hardening, no removal of stains
High Purity: 99.9% v/v ,filtered at 0.2 micron, moisture <0.1%
Universal Compatibility: Works with all automated tissue processors
Cost-Effective: Cheaper than absolute ethanol, ready-to-use
Safe Denaturant System: No methanol toxicity, non-carcinogenic, pleasant odor profile

In Tissue Processing

Step 1: Graded Dehydration :

Fixed tissue contains free and bound water. Alcohol removes it by graded substitution: 70% → 80% → 95% → 100% → 100%. Starting with moderate concentration prevents rapid water removal which can distort delicate cells. Agitation + gentle heat in tissue processors makes it less viscous and increases penetration.

Step 2: Antigen Preservation :

Unlike cross-linking fixatives, alcohol preserve cells through dehydration and protein precipitation. It does NOT mask epitopes.

Step 3: Clearing Transition :

After dehydration, this Alcohol is completely displaced by clearing agents (Xylene / Xylene-free). It leaves no water trace, preventing opaque, poorly infiltrated, hard-to-section blocks.

For Pathologists: Excellent nuclear detail, minimal shrinkage artefacts, no holes/tearing during microtomy, transparent blocks after clearing.
For IHC Lab: Superior HIER response, strong specific staining intensity, reduced background, works for both polymer and biotin systems.
For Technologists: Faster processing, excellent ribboning, compatible with same-day biopsy protocols.
For Lab Managers: One reagent for entire dehydration series, long bath life with anhydrous copper sulfate indicator system, low fire hazard compared to methanol.
Other Compatible Laboratory Uses as Solvent