Ziehl-Neelsen (ZN) Stain Kit
- Ziehl–Neelsen (ZN) Stain Kit is a conventional hot staining system designed for the microscopic demonstration of acid-fast bacilli (AFB) in clinical specimens. It is widely used for the detection of mycobacteria in appropriately prepared smears, particularly in specimens where mycobacterial infection is suspected.
- The method uses heated carbol fuchsin as the primary stain, followed by acid-alcohol decolorization and a contrasting counterstain. Acid-fast organisms retain the primary stain and appear distinctly red against a contrasting background.
- Conventional hot Ziehl–Neelsen staining method
- Designed for demonstration of acid-fast bacilli (AFB)
- Uses heat-assisted carbol fuchsin staining
- Provides strong red staining of acid-fast organisms
- Acid-alcohol differentiation enhances contrast
- Suitable for appropriately prepared clinical smears
- Useful for sputum and other suitable specimen preparations
- Provides clear contrast between AFB and the background
- Suitable for routine microbiology and laboratory microscopy
- Compatible with conventional manual staining procedures
- Acid-fast bacilli possess a cell wall containing a high concentration of mycolic acids and other lipid-rich components, which makes them resistant to decolorization by acid-alcohol after penetration of the primary stain.
- In the hot Ziehl–Neelsen method, strong carbol fuchsin is applied to the smear and gently heated to facilitate penetration of the stain into the waxy bacterial cell wall. After cooling, the smear is treated with an acid-alcohol decolorizer. Acid-fast organisms retain carbol fuchsin, whereas non-acid-fast material is decolorized.
- A counterstain is then applied to provide background contrast. Under light microscopy, acid-fast bacilli appear bright red to pink/red rods against a contrasting blue or otherwise counterstained background.
- Clear AFB Demonstration
- Characteristic Red AFB Reaction
- Enhanced Background Contrast
- Heat-Assisted Staining
- Suitable for Direct Smears
- Useful for Mycobacterial Screening
- Supports Morphological Assessment
- Routine Laboratory Application
- Typical Applications :
- Typical Staining Appearance :
- Provides strong visual staining of acid-fast bacilli for microscopic examination.
- Acid-fast organisms retain carbol fuchsin and appear distinctly red to pink-red after the staining procedure.
- Acid-alcohol differentiation followed by counterstaining helps distinguish AFB from surrounding cellular and specimen material.
- The hot method facilitates penetration of carbol fuchsin into the lipid-rich cell wall of acid-fast organisms.
- Designed for appropriately prepared clinical specimen smears.
- Provides a conventional microscopic method for detecting AFB in specimens submitted for mycobacterial examination.
- Allows assessment of the presence, distribution and approximate morphology of acid-fast organisms.
- Suitable for laboratories performing conventional acid-fast staining and microscopic examination.
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1. AFB examination of sputum smears
2. Respiratory specimen smears
3. Pus and exudate specimens
4. Other appropriately prepared clinical specimens
5. Detection of acid-fast bacilli
6. Conventional mycobacterial microscopy
7. Routine microbiology staining
8. Screening of specimens suspected of containing mycobacteria
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Acid-fast bacilli: Bright red to pink-red rods
Non-acid-fast organisms/background: Take up the counterstain or remain decolorized, depending on the specimen and protocol.
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